Vaults

June 2009 Molecule of the Month by David Goodsell Previous Features
doi: 10.2210/rcsb_pdb/mom_2009_6
Vaults

Our cells are filled with compartments, each performing a specific function. Some of these compartments, such as mitochondria and lysozomes, are very large and enclose many different molecular machines. Other intracellular compartments are smaller, such as the transport vesicles that shuttle proteins from site to site inside the cell. Most of these compartments, including mitochondria, lysozomes and transport vesicles, are surrounded by membranes. However, in special cases, cells build smaller compartments surrounded by a protein shell. In our own cells, vaults are a spectacular example of these protein-enclosed compartments.
Symmetrical Shells

Vaults are composed of many copies of the major vault protein, which assembles to form a hollow football-shaped shell. The one shown here is from rat liver cells (PDB entries 2zuo, 2zv4, and 2zv5) and contains 78 copies of the protein. Inside cells, the vault also encloses a few other molecules, which were not seen in the crystal structure because they don't have a symmetrical structure inside the vault. These molecules include several small RNA molecules, a protein that binds to RNA, and an enzyme that adds nucleotides to proteins.
Mystery of the Vault

Vaults still pose great mysteries. Researchers have been struggling to find out what they do. They are found in many types of cells (each of our own cells contain about 100,000 of them) but some organisms, such as fruit flies and yeast cells, don't have them at all. They are often found in the cytoplasm, where they are transported rapidly from site to site. But, they are also found occasionally in the nucleus, and even more interestingly, they have been found in nuclear pores. These findings, along with lots of other observations, suggest that vaults may be used for transport. Definitive proof, however, still remains to be discovered.

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